Generator

Part:BBa_K2012022

Designed by: Jun Li   Group: iGEM16_HZAU-China   (2016-10-07)


PcpcG2-172-B0034-CheZ-GS3-GFP

This device is composed with a CcaR-P activated promoter which removed a constitutive promoter inside it and a GFP fused CheZ protein which is sensitive in bacterial motility,linked behind it. It can generate CheZ stablely and We can detect the expression of cheZ through the flurescence of GFP. We operate the part in the PJT119b plasmid which comes from Tabor's lab.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1529


[edit]
Categories
Parameters
None